Promoter binding analysis has largely been a laborious and inefficient process. With the advent of “ChIP on chip” (Chromatin Immunoprecipitation on Microarrays or Chips) the research community has been given a new, powerful and high throughput option. This book aims to not only introduce the power of this technology but specifically to provide detailed protocols as to how to perform a ChIP on chip experiment. This book will provide the reader a number of benefits:

• Detailed explanations of the Chromatin Immunoprecipitation technique
• Protocols for performing ChIP on chip experiments
• Informatics approaches to identifying promoter regions in genomic DNA
• Guidelines for analyzing the data that is obtained from a ChIP on chip experiment
• Techniques for methylation analysis of CpG islands using CpG microarrays
• Indications of future directions for improvements to the technology including the development of promoter specific arrays, and regulatory region arrays (enhancers and suppressors).
  

'Microarrays are still predominantly used for gene expression analyses, but they have seen extensive use in genome wide (epigenetic) localization studies. This publication is a great resource for both students and researchers alike working with promotor and cpg island arrays. The chapters are highly informative, focusing on chromatin immunoprecipitation and tiling microarray analysis in both academic and commercial settings. The experimental details and data analysis methods associated with this array format are covered in a comprehensive manner'

Gary Hardiman, Ph.D.

Director BIOGEM, Assistant Professor, Department of Medicine, University of California San Diego

(Editor) Mark Takahashi, Ph.D. is a research scientist at the Microarray Centre (a branch of the Clinical Genomics Centre located at the University Health Network in Toronto, Canada). He obtained his Ph.D. from the Department of Biology at York University (Canada). Currently, he is developing novel technology applications of microarrays to answer specific biological questions. His research interests focus on studying the regulation of gene expression leading to the terminal differentiation and adaptation of skeletal muscle.

(Editor) Neil Winegarden, M.S. is the Head of Operations for the University Health Network Microarray Centre, in Toronto, ON, Canada.  Neil obtained his M.Sc. from the University of Toronto, studying heat shock gene regulation.  Neil was one of the original members of the Microarray Centre, starting in 1998.  Neil’s role is to ensure that the Centre functions on a day to day basis – and he is also responsible for directing technology development efforts.